This essay focuses on a specific carbohydrate. Another way is to write out the methods in detail in either a paragraph form or listed.
Oxidase test 2. BCP Lactose 3. Indole 4. H2S 5. Citrate 6. Motility 7. Methyl Red 8. Urea” Another way is to write out the methods in detail in either a paragraph form or listed. This way is not necessary for this type of paper, since this is lab report for the identification of an unknown bacterium and the methods are explained in detail in the lab manual. If there is a procedure that the instructor added or made changes to, or the student used another procedure not in the course lab manual, then it should be written out and referenced.
identifying an unknown from the web sited below. RESULTS This is where the results are summarized. The method results should be in a table format (see examples below). This is also where the flow chart showing how you arrived at the answer is stated. A short paragraph explaining how carbohydrate.the results are presented can be included. Example: Unknown G had the following morphology on a TSA plate: medium sized opaque cream colored colony. After determining that it was a Gram negative rod, an oxidase test was performed and it was inoculated into a BCP lactose tube and onto a TSA slant. Table I lists all of the biochemical tests, their purpose and results. The results are also shown in a flow chart form. Example: Table 1:
TEST PURPOSE REAGENTS OBSERVATIONS RESULTS Gram stain To determine the Gram reaction of the bacterium Crystal violet, Iodine, Alcohol, Safranin Pink rods Gram negative rods Oxidase test To determine the presence of cytochrome c Oxidase paper Purple / black color change Positive oxidase test BCP Lactose To determine the ability of a bacterium to ferment a specifice None Color change from purple to carbohydrate.yellow Positive lactose fermenter Indole Test To determine the ability of an organism to split indole from tryptophane Kovac’s added to 1 ml of tryptone broth Red Ring at top of broth Positive indole test ETC. Another type of table: Table 1: Physiological and Biochemical Results TEST REAGENTS OR MEDIA TEMP OBSERVATIONS RESULTS INTERPRETATIONS Citrate Citrate slant (green) 350C Color changed from green to blue Positive
citrate as a carbon source Methyl Red MRVP 350C After adding methyl red to one ml, color changed from light yellow to a darker yellow Negative Organism is not able to produce large amounts of acid from glucose fermentation FLOWCHART UNKNOWN G Gram stain Gram negative Rod Oxidase test (positive) Positive Negative Citrobacter freundii Proteus vulgaris Enterobacter aerogenes Proteus mirabilis Escherichia coli Serratia marcescens Klebsiella oxytoca Morganella morganii Klebsiella pneumoniaecarbohydrate. Pseudomonas aeruginosa Pseudomonas aureofaciens Indole test ( Positive) Positive Negative Escherichia
oxytoca Enterobacter aerogenes Klebsiella pneumoniae Citrate Test (negative) Positive Negative Klebsiella oxytoca Escherichia coli Motility Test (positive) Escherichia coli Methyl Red Test (positive) Escherichia coli Unknown B – Escherichia coli DISCUSSION / CONCLUSION This section interprets the meaning of the results.
should be answer here: How did the test result lead to identification? Was it the correct identification? If not, why not. What problems were encounter? This is also where the background information on the organism (environment/pathogenicity) that was identify is mention. Example ofcarbohydrate. a discussion: After several differential tests, it was conclude that unknow G was Escherichia coli. After performing the Gram stain to determine that the unknow was a Gram negative rod, the organism was grow on a TSA slant for use in inoculating the rest of the biochemical tests. All of the biochemical tests work well except for the indole test. It gave a false negative result at first.
since it was inconsistent with the rest of the result. The TA suggest that the test be repeat and it was repeat. The repeated test gave a positive result, consistent with the other data. Therefore it was conclude that the unknown was Escherichia coli. THIS CAN BE ELABORATE MORE FOR EACH TEST. E. coli is in the Enterobacteriaceae family. It is typically found in the human intestines, as well as other animals. It can cause disease in the right host. THE REST OF THIS INFORMATION SHOULD BE RESEARCH FROM THE TEXTBOOK, INTERNET OR OTHER MICROBIOLOGY RESOURCES. REFERENCES:
Firstly, be keen
of references is three, the lab manual,carbohydrate. textbook and atlas. More can be use. Correct reference format must be use. References should be number and the number add to the report when necessary. See example 1 under materials and methods. Spelling of the authors of the references must be correct. Example: 1. De Mers, Marlene. Fundamentals of Microbiology Laboratory Manual, 4 th edition. San Diego: carbohydrate.Montezuma publishing, 2004 2. Tortora, Funk, Case, etc…….. 3. Leboffe, Michael, etc……… 4. http://oregonstate.edu/dept/microbiology/writingmanual/WIC_WritingManual.pdf SOME ADDITIONAL POINTS: 1. Attach both the working worksheet and the final worksheet
identification table that was use for identifying the unknow with the marks use to help narrow down results. 3. Number the pages 4. Use a spell checker 5. Check out the following book from the library: “Successful Lab Reports” a manual for science students by Christopher S. Lobban and Maria Schefter. 6. Use the comments from the instructor for the first report to write the second report even better. 7. Check out the following PDF file on the web: http://oregonstate.edu/dept/microbiology/writingmanual/WIC_WritingManual.pdf
Firstly, be sober
Secondly, be attentive
Lastly, be creative
Lastly, be attentive
finally, be honest
finally, be fast