This essay focuses on determining the Gram reaction. UNKNOWN LETTER (OR NUMBER) YOUR NAME DATE (the due date) LAB INSTRUCTOR
PARTS TO THE UNKNOWN LAB REPORT (Note: Other than the title page, the pages of the report must be numbered) TITLE PAGE There should always be a title page and should include the following information: EXAMPLE OF TITLE PAGE Title should be centered and at the top or in the middle of the page.
UNKNOWN LAB REPORT # 1 This information should be centered and at the bottom part of the title page: UNKNOWN LETTER (OR NUMBER) YOUR NAME DATE (the due date) LAB INSTRUCTOR’S NAME COURSE NAME SEMESTER / YEAR SECTION NUMBER INTRODUCTION This section introduces the reader to the study and why the study was done.
Example: “There are many reasons for knowing the identity of microorganisms. The reasons range from the knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics.
This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium.” MATERIALS AND METHODS This is where the details of the study are listed. Where did the specimen come from, and what methods were used to identify it? Be specific, but do not re write the lab manual.
One way is to mention the names of the materials used and reference the lab manual for the procedure or method and then continue to elaborate when necessary. See example 1. Example 1: “An unknown labeled as letter G was given out by the lab instructor. The methods that have been learned thus far for identifying bacteria have been applied to this unknown. Procedures were followed as stated in the course laboratory manual by De Mers (1), unless otherwise noted. The first procedure that needed to be done was to
Trypticase Soy Agar plate, using the T streak method described in the lab manual.. This needed to be done in order to test the purity of the unknown. After the plates were incubated and grown, the morphology was observed and recorded and a Gram stain was performed. Quality control bacteria were Gram stained along with the unknown to make sure that the Gram stain reaction was done correctly . After determining the Gram reaction, specific biochemical tests were performed. The biochemical tests were
identification tables that were in the lab manual. Since unknown G was determined to be a Gram negative rod, an oxidase test was performed and the organism was inoculated into a BCP lactose tube. Note all of these tests were performed by the methods listed in the lab manual by De Mers (1). Table 1 lists the test, purpose, reagents and results. All of the following tests were performed on this unknown: 1. Oxidase test 2. BCP Lactose 3. Indole 4. H2S 5. Citrate 6. Motility 7. Methyl Red 8. Urea” Another way is to write out th
Firstly, be sure