This essay focuses on test the purity of the unknown. Quality control bacteria were Gram stained along with the unknown
The first procedure that needed to be done was to streak the unknown out on a Trypticase Soy Agar plate, using the T streak method described in the lab manual.. This needed to be done in order to test the purity of the unknown. After the plates were incubated and grown, the morphology was observed and recorded and a Gram stain was performed. Quality control bacteria were Gram stained along with the unknown to make sure that the Gram stain reaction was done correctly .
specific biochemical tests were performed. The biochemical tests were chosen from the unknown identification tables that were in the lab manual. Since unknown G was determined to be a Gram negative rod, an oxidase test was performed and the organism was inoculated into a BCP lactose tube. Note all of these tests were performed by the methods listed in the lab manual by De Mers (1). Table 1 lists the test, purpose, reagents and results.
All of the following tests were performed on this unknown: 1. Oxidase test 2. BCP Lactose 3. Indole 4. H2S 5. Citrate 6. Motility 7. Methyl Red 8. Urea” Another way is to write out the methods in detail in either a paragraph form or listed. This way is not necessary for this type of paper, since this is lab report for the identification of an unknown bacterium and the methods are explained in detail in the lab manual. If there is a procedure that the instructor added or made changes to, or the student used another procedure not in the course lab manual, then it should be written out and referenced. See some of the examples of papers identifying an unknown from the web sited below. RESULTS This is where the results are summarized. The method results should be in a table format (see examples below).
showing how you arrived at the answer is stated. A short paragraph explaining how the results are presented can be included. Example: Unknown G had the following morphology on a TSA plate: medium sized opaque cream colored colony. After determining that it was a Gram negative rod, an oxidase test was performed and it was inoculated into a BCP lactose tube and onto a TSA slant. Table I lists all of the biochemical tests, their purpose and results. The results are also shown in a flow chart form.
firstly, be keen
Thirdly, be cautious