This essay focuses on unknown bacterium and the methods. used another procedure not in the course lab manual, then it should be written out and referenced.
methods in detail in either a paragraph form or listed. This way is not necessary for this type of paper, since this is lab report for the identification of an unknown bacterium and the methods are explaine in detail in the lab manual. If there is a procedure that the instructor added or made changes to, or the student used another procedure not in the course lab manual, then it should be written out and referenced. See some of the examples of papers identifying an unknown from the web sited below. RESULTS This is where the results are summarize.
be in a table format (see examples below). This is also where the flow chart showing how you arrive at the answer is state. A short paragraph explaining how the results are present can be include. Example: Unknown G had the following morphology on a TSA plate: medium size opaque cream colored colony. After determining that it was a Gram negative rod, an oxidase test was perform and it was inoculate into.
This a BCP lactose tube and onto a TSA slant. Table I lists all of the biochemical tests, their purpose and results. The results are also show in a flow chart form. Example: Table 1: Biochemical Test Results TEST PURPOSE REAGENTS OBSERVATIONS RESULTS Gram stain To determine the Gram reaction of the bacterium Crystal violet, Iodine, Alcohol, Safranin Pink rods Gram negative rods Oxidase test To determine the presence of cytochrome c Oxidase paper Purple / black color change Positive oxidase test BCP Lactose.
To determine the ability of a bacterium to ferment a specific carbohydrate None. This Color change from purple to yellow Positive lactose fermenter Indole. Test To determine the ability of an organism to split indole from tryptophane Kovac’s added. This is to 1 ml of tryptone broth Red Ring at top of broth Positive. It is indole test ETC. Another type of table: Table 1:. Physiological and Biochemical Results TEST REAGENTS OR MEDIA TEMP OBSERVATIONS RESULTS INTERPRETATIONS Citrate Citrate slant (green) 350C Color changed from green to blue Positive Organism is able.
as a carbon source Methyl Red MRVP 350C After adding methyl red to one ml. This color changed from light yellow to a darker yellow Negative Organism. This is not able to produce large amounts of acid from glucose. This fermentation FLOWCHART UNKNOWN G Gram stain Gram negative Rod Oxidase test (positive) Positive Negative Citrobacter freundii Proteus vulgaris Enterobacter aerogenes Proteus mirabilis Escherichia coli Serratia marcescens Klebsiella oxytoca Morganella morganii Klebsiella pneumoniae Pseudomonas aeruginosa Pseudomonas aureofaciens.
Firstly, be keen
Secondly, be creative
Thirdly, be innovative
Further, be sober